Protocols


1. Alvetex Scaffold protocols.
2. Alvetex Strata protocols (jump further down this page).

1. Alvetex Scaffold protocols

1.1. Alvetex Scaffold Workflow, Part 1: Preparation, Setup and Seeding

1.1.1. Basic Protocols
PR-SC-01   Quick Start Protocol – Alvetex Scaffold
PR-SC-56   Alvetex Well Inserts – Media Volumes Guide
Alvetex Scaffold in 96-well plates:
PR-SC-02   Quick Start Protocol – Alvetex Scaffold 96-well plate
PR-SC-03   Alvetex Scaffold 96-well plate: Instructions for Use and Sample Applications
Alvetex Scaffold in 384-well plates:
PR-SC-04   Quick Start Protocol – Alvetex Scaffold 384 well plate
PR-SC-05   Alvetex Scaffold 384-well plate: Instructions for Use and Example Application
1.1.2. Alvetex Coating
PR-SC-06   Poly-D or L-lysine Coating of Alvetex Scaffold
PR-SC-07   Collagen I Coating of Alvetex Scaffold
PR-SC-08   Collagen I Thin Gel Coating on Alvetex Scaffold
PR-SC-09   Matrigel™ Coating on Alvetex Scaffold
PR-SC-10   Fibronectin Coating on Alvetex Scaffold
1.1.3. Cell Specific Protocols
PR-SC-11   Guidance notes and Protocols for the culture of Primary rat hepatocytes in well insert and well plate Alvetex Scaffold formats
PR-SC-12   Protocol for the culture of Primary rat hepatocytes in Alvetex Scaffold 96-well plate format
PR-SC-13   Culture of the Canine Kidney Epithelial MDCK Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats
PR-SC-14   Culture of MCF-10A Cell Line on Alvetex Scaffold (12-well insert format, and 96-well plate format)
PR-SC-15   Culture of CaCo-2 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats With or Without a Collagen I Gel Layer
PR-SC-16   Example Protocol for the Co-Culture of the CaCo-2 Cell Line with the CCD-18co Cell Line on Alvetex Scaffold in Well Insert Formats
PR-SC-17   Culture of Breast Carcinoma MDA-MB-231 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats
PR-SC-18   Culture of Human Colon Fibroblastic CCD-18Co Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats
PR-SC-19   Example Protocol for the Culture of the Lung Carcinoma A549 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats
PR-SC-20   Example Protocol for the Culture of the Breast Invasive Ductal Carcinoma Co-Culture BT-474 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats
PR-SC-21   Example Protocol for the Culture of the PC-3 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats
PR-SC-22   Example Protocol for the Culture of the LN-229 Cell Line on Alvetex Scaffold in 96 Well Plate Format
PR-SC-23   Example Protocol for the Culture of the 3T3 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats
PR-SC-24   Example Protocols for the Culture of the TERA2.cl.SP12 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats
PR-SC-25   Example Protocols for the Culture of the HepG2 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats
PR-SC-26   Example Protocols for the Culture of the CHO-K1 Cell Line on Alvetex Scaffold in Well Plate and Well Insert Formats
PR-SC-27   Example Preparation of HaCat cells Epidermal Equivalent Using Alvetex Scaffold
PR-SC-28   Development of Full Thickness Human Skin Model Using Alvetex Scaffold Technology
PR-SC-29   Example Protocol for the Culture of the SW480 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats
PR-SC-30   Example Protocol for the Culture of the SW620 Cell Line on Alvetex Scaffold in Well Insert and Well Plate Formats
PR-SC-31   Example Protocols for the Co-Culture of either the SW620 or the SW480 Cell Line with the 3T3 Cell Line on Alvetex Scaffold in Well Insert Formats
PR-SC-32   Example Protocol for the Culture of the MG63 Cell Line on Alvetex Scaffold in Well Insert Formats
PR-SC-33   Example Protocol for the Culture of Primary Rat MSCs on Alvetex Scaffold in Well Insert Formats
PR-SC-34   Example Protocol for the Culture of the MCF-7 Cell Line on Alvetex Scaffold
1.1.4. Transfection
PR-SC-35   Gene Transfection in 3D – Mirus®

1.2. Alvetex Scaffold Workflow, Part 2: Culturing and Monitoring 3D Cell Growth

1.2.1. Cell Visualisation
PR-SC-36   Light Microscopy – NR-MB Dye
PR-SC-37   Live Cell Imaging of 3D Cultures Grown on Alvetex Scaffold Using Confocal Microscopy
PR-SC-57   Wheat Germ Agglutinin Staining of 3D Cultures Grown on Alvetex Scaffold and Alvetex Strata

Wheat Germ Agglutinin Staining of 3D Cultures Grown on Alvetex Scaffold and Alvetex Strata

1.2.2. Metabolic Assays
PR-SC-38   alamarBlue® Viability Assay
PR-SC-39   PicoGreen Cell Number Assay
PR-SC-40   XTT Cell Viability Assay for Alvetex Scaffold 96-Well Plate Format
PR-SC-41   MTS Viability Assay
PR-SC-42   MTT Viability Assay

1.3. Alvetex Scaffold Workflow, Part 3: Imaging, Harvesting and Downstream Analysis

1.3.1. Genomics and Proteomics
PR-SC-43   Total Protein Extraction from Cells Cultured in Alvetex Scaffold in 3D
PR-SC-44   Protein Extraction from Cells Cultured in Alvetex Scaffold 96-Well Plate Format
PR-SC-45   Extraction of RNA from Cells Cultured in Alvetex Scaffold in 3D
PR-SC-46   RNA Extraction from Cells Cultured in Alvetex Scaffold 96-Well Plate Format
1.3.2. Histology
PR-SC-47   Histology Series Part 1. Choosing the Right Fixative to Preserve 3D Cell Cultures
PR-SC-48   Histology Series Part 2. Processing Alvetex Scaffold 3D Cultures for PFA Fixation and Paraffin Wax Embedding for Immunofluorescence
PR-SC-49   Histology Series Part 3. Processing Alvetex Scaffold 3D Cultures for Bouin’s Fixation and Paraffin Wax Embedding for Haematoxylin and Eosin Staining
PR-SC-50   Histology Series Part 4. Processing Alvetex Scaffold 3D Cultures for Resin Embedding and Toluidine Blue Staining for Light Microscopy
PR-SC-51   Histology Series Part 5. Processing Alvetex Scaffold 3D Cultures for Scanning Electron Microscopy (SEM)
1.3.3. Advanced Microscopy

See also Histology Series Part 5, above.

PR-SC-52   Processing Alvetex Scaffold 3D Cultures for Cryosectioning
PR-SC-53   Immunofluorescence Confocal Microscopy of 3D Cultures Grown on Alvetex Scaffold
1.3.4. Cell Retrieval
PR-SC-54   Retrieval of Cells from Alvetex Scaffold
PR-SC-55   Protocol for the removal of Alvetex discs from 6-well or 12-well inserts by unclipping the well insert base

2. Alvetex Strata protocols

PR-ST-01   Quick Start Protocol – Alvetex Strata
PR-ST-02   Retrieval of Cells from Alvetex Strata
PR-SC-57   Wheat Germ Agglutinin Staining of 3D Cultures Grown on Alvetex Scaffold and Alvetex Strata