Alvetex 3D Cell Culture Systems


Alvetex is a highly porous polystyrene scaffold designed for 3D cell culture.

Cells grown in Alvetex maintain their in vivo morphology, behaviour and responsiveness within an model system. Alvetex enables cells to maintain their natural shape and to freely interact with neighbouring cells in 3D so that they function in a more physiologically relevant manner.

Presented as a 200 μm thick membrane, Alvetex has been adapted to fit a variety of conventional cell culture plasticware formats. Each product unit has been terminally sterilised by gamma irradiation and remains sterile until its blister pack is opened. Alvetex requires an ethanol wash prior to use to render it hydrophilic. Alvetex does not degrade during normal use.

 Download the Alvetex 3D Cell Culture Systems catalog

Alvetex distributors worldwide

 

Different Alvetex Architectures to suit your research needs

Alvetex is now available in two types: Alvetex Scaffold and Alvetex Strata. Both materials are presented as 200 μm thick membranes of highly porous cross-linked polystyrene. The difference between them is their fine structure and architecture.


Alvetex Scaffold, our market leading product, is primarily designed for three dimensional culture of dissociated mammalian cells within the scaffold. Average void size: 42 μm.

Scanning electron micrograph of Alvetex Scaffold in transverse section. Scale bar 100 μm.

 

Alvetex Strata, our second generation product, is primarily designed to support the growth of cells and intact tissues on the surface of the membrane. Average void size: 15 μm.

Scanning electron micrograph of Alvetex Strata in transverse section. Scale bar 100 μm.

 

Multiwell Plate Formats: Alvetex Scaffold

Alvetex Scaffold 12 Well Plate

Comprised of a single loose disc of Alvetex Scaffold and a polystyrene clip in each well of a 12 well plate. The clip holds the disc in position during transit and use, and can easily be removed for access to the Alvetex Scaffold and cells grown in 3D culture.

The 12 well plate format, a simple presentation of Alvetex Scaffold technology, is primarily suitable for short term culture experiments where the medium is replaced every 1-2 days.



Product Catalogue code Presentation
Alvetex® Scaffold 12 Well Plate (with lid) AVP002-2 2 × 12 well plates
AVP002-10 10 × 12 well plates
AVP002-80 80 × 12 well plates

Note: Plates are individually sterile blister packed.


Alvetex Scaffold 24 Well Plate

Comprised of a single loose disc of Alvetex Scaffold and a polystyrene clip in each well of a 24 well plate. The clip holds the disc in position during transit and use, and can easily be removed for access to the Alvetex Scaffold and cells grown in 3D culture.

The 24 well plate format, a simple presentation of Alvetex Scaffold technology, is primarily suitable for short term culture experiments where the medium is replaced every 1-2 days.



Product Catalogue code Presentation
Alvetex® Scaffold 24 Well Plate (with lid) AVP006-2 2 × 24 well plates
AVP006-10 10 × 24 well plates
AVP006-80 80 × 24 well plates

Note: Plates are individually sterile blister packed.


Alvetex Scaffold 96 Well Plate

Comprised of a black 96 well plate, clear plastic base, with Alvetex Scaffold at the bottom of each well. The Alvetex Scaffold has been heat welded to the base of the wells in a process which does not alter its physical structure.

Cells growing in 3D are exposed to culture medium from above only, and therefore predominantly reside in the top portion of scaffold.

Alvetex Scaffold 96 well plate technology is compatible with a wide range of in vitro cell viability assays.



Product Catalogue code Presentation
Alvetex® Scaffold 96 Well Plate (with lid) AVP009-2 2 × 96 well plates
AVP009-10 10 × 96 well plates
AVP009-80 80 × 96 well plates

Note: Plates are individually sterile blister packed.


Alvetex Scaffold 384 Well Plate

Comprised of a black 384 well plate, clear plastic base, with Alvetex Scaffold at the bottom of each well. The Alvetex Scaffold has been heat welded to the base of the wells in a process which does not alter its physical structure.

Cells growing in 3D are exposed to culture medium from above only, and therefore predominantly reside in the top portion of scaffold.

Alvetex Scaffold 384 well plate technology is compatible with a wide range of in vitro cell viability assays.



Product Catalogue code Presentation
Alvetex® Scaffold 384 Well Plate (with lid) AVP009-2 2 × 384 well plates
AVP009-10 10 × 384 well plates
AVP009-80 80 × 384 well plates

Note: Plates are individually sterile blister packed.


Well Insert Formats: Alvetex Scaffold and Alvetex Strata

Alvetex 6 Well Insert Format

Comprised of discs of either Alvetex Scaffold or Alvetex Strata in individually sealed polystyrene inserts, designed to fit into most 6 well plates or our custom-made “Alvetex Well Insert Holder in Deep Petri Dish” (AVP015).

Note that plates and well insert holders are not supplied with the product and have to be sourced separately.

The presentation of Alvetex in well insert formats is versatile, enabling long term 3D culture as cells can receive nutrients from media above and below the membrane, sustaining optimal 3D cell growth.



Product Catalogue code Presentation
Alvetex® Scaffold 6 well inserts AVP004-12 12 inserts
AVP004-48 48 inserts
AVP004-96 96 inserts
Alvetex® Strata 6 well inserts STP004-12 12 inserts
STP004-48 48 inserts
STP004-96 96 inserts

Note: Inserts are individually sterile blister packed.


Alvetex Well Inserts enable three different media fill options

1. Media in contact from below only.

This enables 3D growth at the air/ liquid interface.

2. Media in contact above and below.

Independent compartments enable 3D growth with two different media constituents.

3. Media in contact above and below.

Interconnected compartments enable optimal conditions for maximising cell growth and increased viability.


Alvetex 12 Well Insert Format

Comprised of discs of either Alvetex Scaffold or Alvetex Strata in individually sealed polystyrene inserts, designed to fit into most 6 well plates or our custom-made “Alvetex Well Insert Holder in Deep Petri Dish” (AVP015).

Snapping the extended wings off Alvetex 12 well inserts (along “break lines”) enables fitting into 12 well plates.

Note that plates and well insert holders are not supplied with the product and have to be sourced separately.

The presentation of Alvetex in well insert formats is versatile, enabling long term 3D culture as cells can receive nutrients from media above and below the membrane, sustaining optimal 3D cell growth.



Product Catalogue code Presentation
Alvetex® Scaffold 12 well inserts AVP005-12 12 inserts
AVP005-48 48 inserts
AVP005-96 96 inserts
Alvetex® Strata 12 well inserts STP005-12 12 inserts
STP005-48 48 inserts
STP005-96 96 inserts

Note: Inserts are individually sterile blister packed.


Alvetex Tools

Alvetex Well Insert Holder and Deep Petri Dish

Comprised of a single well insert holder in a deep Petri dish with lid. The well insert holder is capable of housing up to three Alvetex well inserts (either 6 or 12 well inserts). The Petri dish itself is not tissue culture treated.

The Alvetex Well Insert Holder and Deep Petri Dish enables users to grow their 3D cultures in larger volumes of media compared to an ordinary multiwell plate, facilitating fewer media changes. Capable of sustaining long term 3D culture experiments (3-4 weeks).

The well insert can be positioned at three different levels in the insert holder: high, medium and low. This feature allows cultures to be raised to the air liquid interface by moving the insert to a different level within the same holder.

Positioning the well inserts at different levels may also be used to conserve expensive media or allow for increasing media volumes for demanding cell types over the course of a long term experiment.



Product Catalogue code Presentation
Alvetex® Well Insert Holder and Deep Petri Dish (with lid) AVP015-12 2 units
AVP015-10 10 units

Note: Units are individually sterile blister packed.


Alvetex Well Insert Holder media fill options

The Alvetex Well Insert Holder can hold Alvetex Well Inserts at different levels in the culture medium

1. Well insert on upper level.

2. Well insert on middle level.

3. Well insert on lower level.


Alvetex Perfusion Plate

This product allows scientists to create cell based models that are another step closer to the environment experienced by cells and tissue in vivo. The systems can also be used to create complex co-cultures, multi-organ systems and to study paracrine effects.

Each unit contains an Alvetex Perfusion Plate with a lid and two Luer locks.

Example: Alvetex Perfusion Plate showing 4 × Alvetex 12 Well Inserts in position



Product Catalogue code Presentation
Alvetex® Perfusion Plate (with lid) AVP011-12 2 units
AVP011-10 10 units

Note: Units are individually sterile blister packed. Pump and tubing is not included.


Alvetex Kits

Product Catalogue code Presentation
Alvetex® Scaffold Plate Starter Kit AVP-KIT-1 1 × 12 well plate

1 × 24 well plate

1 × 96 well plate
Alvetex® Scaffold Well Insert Starter Kit AVP-KIT-2 6 × 6 well inserts

6 × 12 well inserts

1 × Alvetex Well Insert Holder in a deep Petri dish
Alvetex® Strata Well Insert Starter Kit STP-KIT-2 6 × 6 well inserts

6 × 12 well inserts

1 × Alvetex Well Insert Holder in a deep Petri dish
Kit: Alvetex® Perfusion Plate with

Alvetex® Scaffold 6 well inserts
AVP-KIT-3 2 × Alvetex Perfusion Plates with Luer locks

12 × Alvetex Scaffold 6 well inserts
Kit: Alvetex® Perfusion Plate with

Alvetex® Scaffold 12 well inserts
AVP-KIT-4 2 × Alvetex Perfusion Plates with Luer locks

12 × Alvetex Scaffold 12 well inserts
Kit: Alvetex® Perfusion Plate with

Alvetex® Scaffold 6 well inserts
AVP-KIT-5 5 × Alvetex Perfusion Plates with Luer locks

48 × Alvetex Scaffold 6 well inserts
Kit: Alvetex® Perfusion Plate with

Alvetex® Scaffold 12 well inserts
AVP-KIT-6 5 × Alvetex Perfusion Plates with Luer locks

48 × Alvetex Scaffold 12 well inserts

Note: Units are individually sterile blister packed. Pump and tubing is not included in Alvetex Perfusion Plate kits.


Ordering information

The full range of Alvetex products are sold through various scientific lab-ware distributors around the world.

Alvetex distributors worldwide