3D Learning Zone


This is the place to learn about  using Alvetex 3D cell culture technology.

Promotional Videos

 

What is Real 3D Cell Culture?

Comparison of 3D Cell Culture Techniques

Instructional Videos

 

Choosing the right format to suit your needs

How to prepare and handle Alvetex Scaffold

Seeding cells on to Alvetex Scaffold


Alvetex Protocols

We have a growing collection of protocols for various aspects of cell culture using Alvetex Scaffold which we have organised into categories following the typical experimental procedure:

Workflow 1: Preparation, Setup & Seeding

  • Experimental design
  • Choosing cells media and supplements
  • Activation of Alvetex Scaffold
  • Use of Coating agents with Alvetex Scaffold
  • Cell seeding & recommended seeding density

Workflow 2: Culturing & Monitoring 3D cell growth

  • Monitor / optimise cell attachment/growth
  • Visualisation throughout experimental time-course
  • Carrying out required media changes
  • Metabolic assays

Workflow 3: Imaging, Harvesting & Downstream Analysis

  • Histology
  • Immunofluorescence
  • Confocal microscopy
  • SEM and TEM
  • End-point analysis
  • Cell Retrieval
  • Retrieval of RNA and Protein

A full list of our protocols is available on our Protocols page.

You may also be interested in viewing our White Papers and Application Notes.