Science Portal: Perfused 3D cell culture


Featured Application note

AN-SC-08 – Reinnervate Perfusion Plate: Dynamic Circulation and Perfusion of Culture Medium Within a Multi-welled Plate

Much of our current understanding of molecular and cellular biology has been generated using static cultures of cells grown in artificial monolayers. With the introduction of products such as Alvetex Scaffold, it is now practical to grow cells in 3D to better mimic the way cells grow in vivo surround by other cells and ECM proteins.

Here we introduce a simple, low-cost perfusion system that can combine 3D cell culture with dynamic circulation and perfusion of cell culture media. This product allows scientists to create cell based models that are another step closer to the environment experienced by cells and tissue in vivo. The systems can also be used to create complex co-cultures, multi organ systems and to study paracrine effects.

The Reinnervate perfusion system is compatible with the following methods:

  • 3D cell culture using Alvetex well insert technology
  • 3D co-culture using Alvetex well insert technology
  • Standard 2D cell culture
  • Hydrogel-based 3D cell cultures
  • Transwell and well insert technology from various suppliers

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Ordering information

Alvetex Perfusion Plates are available in the following kits:

Product Code Description
AVP011-2 Perfusion plate

Pack of 2 plates and Luer locks

(Pump and tubing not included)

AVP011-10 Perfusion plate

Pack of 10 plates and Luer locks(Pump and tubing not included)

AVP-KIT-3 Perfusion plate with Alvetex Scaffold 6-well inserts

Pack of 2 plates and Luer locks, plus 12 Alvetex Scaffold 6-well inserts

(Pump and tubing not included)

AVP-KIT-4 Perfusion plate with Alvetex Scaffold 12-well inserts

Pack of 2 plates and Luer locks, plus 12 Alvetex Scaffold 12-well inserts

(Pump and tubing not included)

AVP-KIT-5 Perfusion plate with Alvetex® Scaffold 6-well inserts

Pack of 5 plates and Luer locks, plus 48 Alvetex Scaffold 6-well inserts

(Pump and tubing not included)

AVP-KIT-6 Perfusion plate with Alvetex Scaffold 12-well inserts

Pack of 5 plates and Luer locks, plus 48 Alvetex Scaffold 12-well inserts

(Pump and tubing not included)

All Reinnervate products are sold through various scientific labware distributors around the world.

Find a distributor for your country »