Science Portal: Skin


Featured Application Note

Three Dimensional Culture of HaCaT Keratinocytes Using Alvetex Scaffold

Its upper most layer, the stratum corneum, provides the principal barrier of the skin. The stratum corneum consists of flat, dead keratinocytes (corneocytes) surrounded by a lipid bilayer matrix, which forms a dense and compact structure often described as “brick and mortar”. The corneocytes are filled with keratin filaments, which are surrounded by a densely cross-linked protein layer, the cell envelope. A lipid envelope is chemically linked to the densely packed cell envelope. This lipid monolayer provides the interface between the hydrophilic corneocytes and the lipophilic non-polar lipids which are surrounding the corneocytes. The corneocytes are interconnected by corneo-desmosomes which are important for stratum corneum cohesion. The change in lipid composition and cell structure during terminal differentiation produces a very densely packed structure in the stratum corneum. This impermeable nature of the cornified envelope is what helps direct the penetration of substances between the corneocytes.

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AN-SC-13   Three Dimensional Culture of HaCaT Keratinocytes Using Alvetex Scaffold
Related Protocols

PR-SC-28 – Development of Full Thickness Human Skin Model Using Alvetex Scaffold Technology

PR-SC-27 – Example Preparation of HaCat cells Epidermal Equivalent Using Alvetex Scaffold

Ordering information

Alvetex Scaffold is currently available in the following formats. The formats relevant to skin cell culture are highlighted.

Product Catalogue code Presentation
Alvetex Scaffold Plate Starter Pack AVP-KIT-1 1 x 12 well plate
1 x 24 well plate
1 x 96 well plate
– all individually sterile blister packed
Alvetex Scaffold Well Insert Starter Pack AVP-KIT-2 6 x 6 well inserts
6 x 12 well inserts
1 x deep petri-dish containing a well insert holder
– all individually sterile blister packed
Alvetex Scaffold 12 well plate AVP002-2 2 x 12 well plates, individually sterile blister packed
Alvetex Scaffold well insert holder and deep petri-dish AVP015-2 2 units, individually sterile blister packed
Alvetex Scaffold 6 well insert AVP004-12 12 inserts, individually sterile blister packed
Alvetex Scaffold 12 well insert AVP005-12 12 inserts, individually sterile blister packed
Alvetex Scaffold 24 well plate AVP006-2 2 x 24 well plates, individually sterile blister packed
Alvetex Scaffold 96 well plate AVP009-2 2 x 96 well plates, individually sterile blister packed

Alvetex Scaffold is sold through various scientific labware distributors around the world.

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