Science Portal: Stem Cells
Featured Application Notes
AN-ST-02 – Propagation of Human Pluripotent Stem Cells in Three Dimensional Culture Using Alvetex Strata
We have developed a strategy that enables the continuous propagation and maintenance of cells in three-dimensional (3D) culture. The technology relies on culturing cells continuously on the surface of Alvetex Strata, a highly porous membrane presented in a well insert format. As proof of concept, we demonstrate the propagation of human pluripotent stem cells in 3D over many passages. We show how these stem cells adapt to their surroundings and acquire a more 3D shape. Such cells appear to become ‘primed’ for growth in 3D and consequently show marked improvements in their developmental potential when subsequently grown in a range of established 3D cell differentiation models.
This application note demonstrates how using Alvetex Strata offers a new approach to cultivating cells continuously in 3D to achieve notable improvements in cell growth and function. It can be used to routinely maintain proliferative cells with a 3D structural phenotype in preparation for their use in down-stream assays that involve 3D cell growth.
AN-SC-01 – Formation of Mesenchymal Tissues in Alvetex Scaffold Derived From Stem Cells and Established Cell Lines
Based on an increasing body of evidence, it is generally accepted that culturing cells in three dimensional (3D) models can lead to superior cell viability, differentiation and function compared to existing conventional culture systems where cells largely grow as monolayers on two dimensional (2D) substrates. These enhanced qualities are important for the retention of the in vivo-like cell phenotype, cell differentiation potential and subsequent functionality as required for downstream applications. Here we demonstrate the application of Alvetex Scaffold technology for the growth and differentiation of mesenchymal cells and the formation of differentiated tissues.
Ordering information
Alvetex products are currently available in the following formats. The formats relevant to stem cell culture are highlighted.
Alvetex Strata
| Well Insert Formats Inserts are individually sterile blister packed |
||
| Alvetex Strata 6 well insert | STP004-12 | 12 inserts |
| STP004-48 | 48 inserts | |
| STP004-96 | 96 inserts | |
| Alvetex Strata 12 well insert | STP005-12 | 12 inserts |
| STP005-48 | 48 inserts | |
| STP005-96 | 96 inserts | |
Alvetex Scaffold
| Product | Catalogue code | Presentation |
|---|---|---|
| Alvetex Scaffold Plate Starter Pack | AVP-KIT-1 | 1 x 12 well plate 1 x 24 well plate 1 x 96 well plate – all individually sterile blister packed |
| Alvetex Scaffold Well Insert Starter Pack | AVP-KIT-2 | 6 x 6 well inserts 6 x 12 well inserts 1 x deep petri-dish containing a well insert holder – all individually sterile blister packed |
| Alvetex Scaffold 12 well plate | AVP002-2 | 2 x 12 well plates, individually sterile blister packed |
| Alvetex Scaffold well insert holder and deep petri-dish | AVP015-2 | 2 units, individually sterile blister packed |
| Alvetex Scaffold 6 well insert | AVP004-12 | 12 inserts, individually sterile blister packed |
| Alvetex Scaffold 12 well insert | AVP005-12 | 12 inserts, individually sterile blister packed |
| Alvetex Scaffold 24 well plate | AVP006-2 | 2 x 24 well plates, individually sterile blister packed |
| Alvetex Scaffold 96 well plate | AVP009-2 | 2 x 96 well plates, individually sterile blister packed |
Alvetex is sold through various scientific labware distributors around the world.
