Science Portal: Stem Cells


Featured Application Notes

AN-ST-02 – Propagation of Human Pluripotent Stem Cells in Three Dimensional Culture Using Alvetex Strata

We have developed a strategy that enables the continuous propagation and maintenance of cells in three-dimensional (3D) culture. The technology relies on culturing cells continuously on the surface of Alvetex Strata, a highly porous membrane presented in a well insert format. As proof of concept, we demonstrate the propagation of human pluripotent stem cells in 3D over many passages. We show how these stem cells adapt to their surroundings and acquire a more 3D shape. Such cells appear to become ‘primed’ for growth in 3D and consequently show marked improvements in their developmental potential when subsequently grown in a range of established 3D cell differentiation models.

This application note demonstrates how using Alvetex Strata offers a new approach to cultivating cells continuously in 3D to achieve notable improvements in cell growth and function. It can be used to routinely maintain proliferative cells with a 3D structural phenotype in preparation for their use in down-stream assays that involve 3D cell growth.

AN-SC-01 – Formation of Mesenchymal Tissues in Alvetex Scaffold Derived From Stem Cells and Established Cell Lines

Based on an increasing body of evidence, it is generally accepted that culturing cells in three dimensional (3D) models can lead to superior cell viability, differentiation and function compared to existing conventional culture systems where cells largely grow as monolayers on two dimensional (2D) substrates. These enhanced qualities are important for the retention of the in vivo-like cell phenotype, cell differentiation potential and subsequent functionality as required for downstream applications. Here we demonstrate the application of Alvetex Scaffold technology for the growth and differentiation of mesenchymal cells and the formation of differentiated tissues.

Ordering information

Alvetex products are currently available in the following formats. The formats relevant to stem cell culture are highlighted.

Alvetex Strata
Well Insert Formats
Inserts are individually sterile blister packed
Alvetex Strata 6 well insert STP004-12 12 inserts
STP004-48 48 inserts
STP004-96 96 inserts
Alvetex Strata 12 well insert STP005-12 12 inserts
STP005-48 48 inserts
STP005-96 96 inserts
Alvetex Scaffold
Product Catalogue code Presentation
Alvetex Scaffold Plate Starter Pack AVP-KIT-1 1 x 12 well plate
1 x 24 well plate
1 x 96 well plate
– all individually sterile blister packed
Alvetex Scaffold Well Insert Starter Pack AVP-KIT-2 6 x 6 well inserts
6 x 12 well inserts
1 x deep petri-dish containing a well insert holder
– all individually sterile blister packed
Alvetex Scaffold 12 well plate AVP002-2 2 x 12 well plates, individually sterile blister packed
Alvetex Scaffold well insert holder and deep petri-dish AVP015-2 2 units, individually sterile blister packed
Alvetex Scaffold 6 well insert AVP004-12 12 inserts, individually sterile blister packed
Alvetex Scaffold 12 well insert AVP005-12 12 inserts, individually sterile blister packed
Alvetex Scaffold 24 well plate AVP006-2 2 x 24 well plates, individually sterile blister packed
Alvetex Scaffold 96 well plate AVP009-2 2 x 96 well plates, individually sterile blister packed

Alvetex is sold through various scientific labware distributors around the world.

Find a distributor for your country »