A list of Publications in Scientific Journals detailing research where Alvetex® has been used

The publications below are hosted on the websites of various scientific journals. Clicking a link will open the PDF in a new window / tab.

Development of positive control tissue for in situ hybridisation using Alvetex scaffolds

Christa MacDonald, David Benjamin Finlay, Anower Jabed, Michelle Glass, E. Scott Graham

Journal of Neuroscience Methods

Published 30 December 2014

In this study, the authors present a new method to generate biological samples that can be used as controls for in situ hybridisation (ISH) studies. Currently, ISH is limited due to inefficient probe penetration and loss of sample during preparation. In this paper researchers describe a novel approach using Alvetex Scaffold to create 3D tissue-like structures suitable for sectioning on a cryostat. These samples can subsequently be used as material for positive controls to verify gene expression by ISH. Cells are first transfected with the gene of interest, then cultured in 3D on Alvetex Scaffold, samples are then fixed, embedded and sectioned, and subsequently prepared for ISH using the riboprobe of interest. Sectioning increases probe penetration and further enhances the opportunity for good hybridisation. The authors demonstrated this technique using HEK cells transfected with CB1 and NeuN to optimise hybridisation stringency conditions. These conditions were then applied to samples of brain tissue analysed by ISH. This method can be adapted to generate positive controls for ISH for any gene of interest and it is especially useful where access to precious tissue is limited. In relation to Alvetex, this method further demonstrates the versatility of the material and its broad range of applications.

Topographical cues regulate the crosstalk between MSCs and macrophages

Gema Vallésa, Fátima Bensiamara, Lara Crespoa, Manuel Arruebob, Nuria Vilaboaa, Laura Saldañaa

DOI: 10.1016/j.biomaterials.2014.10.028

Published online October 28, 2014

Valles and colleagues report on the role of the physical microenvironment in the modulation of signalling between mesenchymal stem cells and macrophages. Three dimensional culture of mesenchymal stem cells in Alvetex Scaffold stimulates the secretion of anti-inflammatory molecules that have a differential effect on co-cultured macrophages compared to cells grown in conventional cell culture on flat two dimensional substrates. The role of specific factors involved in this signalling process, namely IL-6 and MCP-1, was identified using immunological inhibition. In effect, the local inflammatory environment provided in 3D co-cultures induces a decrease in monocyte migration compared conventional monolayer cultures. These data highlight the importance of the three dimensional topography of the microenvironment in the regulation of paracrine factors and soluble-factor guided communication between two different cell populations.

Pan-Bcl-2 Inhibitor Obatoclax Delays Cell Cycle Progression and Blocks Migration of Colorectal Cancer Cells

Bruno Christian Koehler,  Anna-Lena Scherr,  Stephan Lorenz,  Christin Elssner,  Nicole Kautz,  Stefan Welte,  Dirk Jaeger,  Toni Urbanik,  Henning Schulze-Bergkamen

PLoS ONE 9(9): e106571.
DOI: 10.1371/journal.pone.0106571

Published September 05, 2014

Morphology and functions of astrocytes cultured on water-repellent fractal tripalmitin surfaces

Wei-wei Hu, Zhe Wang, Shan-shan Zhang, Lei Jiang, Jing Zhang, Xiangnan Zhang, Qun-fang Lei, Hyun-Joo Park, Wen-jun Fang, Zhong Chen

Biomaterials 2014
DOI: 10.1016/j.biomaterials.2014.05.026

Published 2 June 2014

Note: Hu and colleagues present work that describes the development and application of water-repellent fractal tripalmitin surfaces upon which they cultured primary rat astrocytes. Cells grown on these substrates form a more three dimensional in vivo-like phenotype. Such cell architecture was confirmed in comparison to the cells growing on Alvetex® Scaffold. In this case, Alvetex Scaffold was used as a form of positive control to create a three dimensional micro-environment in which the astrocytes could form in vivo-like morphologies. When cultured on Alvetex Scaffold, the astrocytes developed a more sophisticated structure: they formed more numerous and longer processes, they developed numerous filopodia extensions, and they increased cell-to-cell interactions. The paper also demonstrates the use of confocal microscopy to image the GFAP-immunostained astrocytes growing in Alvetex Scaffold.


The role of high cell density in the promotion of neuroendocrine transdifferentiation of prostate cancer cells

Zuzana Pernicová, Eva Slabáková, Radek Fedr, Šárka Šimečková, Josef Jaroš, Tereza Suchánková, Jan Bouchal, Gvantsa Kharaishvili, Milan Král, Alois Kozubík and Karel Souček

Molecular Cancer 2014, 13:113

Published: 20 May 2014

The effects of microRNAs on human neural stem cell differentiation in two- and three-dimensional cultures

Lara Stevanato and John D Sinden

Stem Cell Research & Therapy 2014, 5:49

Published: 11 April 2014

Small molecule phosphorescent probes for O2 imaging in 3D tissue models

Ruslan I. Dmitriev, Alina V. Kondrashina, Klaus Koren, Ingo Klimant, Alexander V. Zhdanov, Janelle M. P. Pakan, Kieran W. McDermott and Dmitri B. Papkovsky

Biomaterials Science (28 Jan 2014)
DOI: 10.1039/c3bm60272a

Beyond Cell Death – Antiapoptotic Bcl-2 Proteins Regulate Migration and Invasion of Colorectal Cancer Cells In Vitro

Bruno Christian Koehler, Anna-Lena Scherr, Stephan Lorenz, Toni Urbanik, Nicole Kautz, Christin Elssner, Stefan Welte, Justo Lorenzo Bermejo, Dirk Jäger, Henning Schulze-Bergkamen

Public Library of Science.
PLoS ONE 8(10): e76446.

Effect of PEG Surface Conformation on Anticancer Activity and Blood Circulation of Nanoemulsions Loaded with Tocotrienol-Rich Fraction of Palm Oil

Alaadin Alayoubi, Saeed Alqahtani, Amal Kaddoumi, Sami Nazzal

The AAPS Journal (August 2013)
DOI: 10.1208/s12248-013-9525-z

Overlapping gene coexpression patterns in human medullary thymic epithelial cells generate self-antigen diversity

Sheena Pinto, Chloé Michela, Hannah Schmidt-Glenewinkel, Nathalie Harder, Karl Rohr, Stefan Wild,
Benedikt Brorse, and Bruno Kyewskia,

PNAS. 26 August 2013. DOI: 10.1073

Epidermal-like architecture obtained from equine keratinocytes in three-dimensional cultures

Ruchi Sharma, Safia Z. Barakzai, Sarah E. Taylor, F. Xavier Donadeu

Journal of Tissue Engineering and Regenerative Medicine.

First published online 30 July 2013. DOI: 10.1002/term.1788

Targeting of Beta Adrenergic Receptors Results in Therapeutic Efficacy against Models of Hemangioendothelioma and Angiosarcoma

Jessica M. Stiles, Clarissa Amaya, Steven Rains, Dolores Diaz, Robert Pham, James Battiste, Jaime F. Modiano, Victor Kokta, Laura E. Boucheron, Dianne C. Mitchell, Brad A. Bryan

Public Library of Science.
March 2013 | Volume 8 | Issue 3 | e60021
PLoS ONE 8(3): e60021.

Characterization of liver specific functions of upcyte® hepatocytes grown in 3D and co-cultured with upcyte® endothelial cells

C Dähn, N Hewitt, D Maltman, G Talas, S Przyborski, S Heinz, A Nörenberg, K Scheller, J Braspenning

Z Gastroenterol, 2012; 50 – P2_05
DOI: 10.1055/s-0031-1295802

The autophagy-associated factors DRAM1 and p62 regulate cell migration and invasion in glioblastoma stem cells

S Galavotti, S Bartesaghi, D Faccenda, M Shaked-Rabi, S Sanzone, A McEvoy, D Dinsdale, F Condorelli, S Brandner, M Campanella, R Grose, C Jones, P Salomoni

Oncogene, 2012, doi: 10.1038/ocn.2012.111

Generation of proliferating human hepatocytes using upcyte® technology: characterisation and applications in induction and cytotoxicity assays

Alexandra Burkard, Caroline Dähn, Stefan Heinz, Anne Zutavern, Vera Sonntag-Buck, Daniel Maltman, Stefan Przyborski, Nicola J. Hewitt, and Joris Braspenning

Xenobiotica, 2012,doi: 10.3109/00498254.2012.675093

Generation of proliferating human hepatocytes using upcyte® technology: characterisation and applications in induction and cytotoxicity assays

Alexandra Burkard1, Caroline Dähn, Stefan Heinz, Anne Zutavern, Vera Sonntag-Buck, Daniel Maltman, Stefan Przyborski, Nicola J. Hewitt, Joris Braspenning

General Xenobiochemistry, October 2012, Vol. 42, No. 10 , Pages 939-956

Rat primary hepatocytes show enhanced performance and sensitivity to acetaminophen during three-dimensional culture on a polystyrene scaffold designed for routine use.

Schutte, M., Fox. B., Baradez, M., Devonshire, A., Minguez., J., Bokhari. M., Przyborski. S., Marshall. D., (2011)

Assay and Drug Development Technologies

DOI: 10.1089/adt.2011.0371

Adipose tissue-derived stem cells display a proangiogenic phenotype on 3D scaffolds.

Neofytou. E.A., Chang. E., Patlola. B., Joubert. L.M., Rajadas. J., Gambhir. S.S., Cheng. Z., Robbins. R.C., Beygui. R.E., (2011)
Journal Biomedical Materials Research Part A

DOI: 10.1002/jbm.a.33113

Dysregulated TRK signalling is a therapeutic target in CYLD defective tumours.

Rajan. N., Elliott. R., Clewes. O., Mackay. A., Reis-Filho. J.S., Burn. J., Langtry. J., Sieber-Blum. M., Lord. C.J., Ashworth. A., (2011)

DOI:  10.1038/onc.2011.133

Alvetex: Polystyrene scaffold technology for routine three dimensional cell culture.

Knight, E., Murray, B., Carnachan, R., Przyborski, S.A., (2011).
Methods in Molecular Biology, 695, 323-40.

Developments in three dimensional cell culture technology aimed at improving the accuracy of in vitro analyses.

Maltman, D., Przyborski, S.A., (2010)
Biochemical Society Transactions, 38(4), 1072-5


Emulsion-templated porous scaffolds enabling three dimensional cell culture. Polymer Preprints

Carnachan, R.J., Bokhari, M., Maatta, A., Cameron, N.R., Przyborski, S.A. (2008).
American Chemical Society, Division of Polymer Chemistry, 49, 418-419.

Effect of synthesis parameters on emulsion-templated porous polymer formation and evaluation for 3D cell culture scaffolds.

Bokhari, M., Carnachan, R., Przyborski, S.A., Cameron, N.R. (2007).
Journal of Materials Chemistry, 17, 4088-4094.

Culture of HepG2 liver cells on three dimensional polystyrene scaffolds enhances cell structure and function during toxicological challenge.

Bokhari, M., Carnachan, R., Cameron, N.R., Przyborski, S.A. (2007).
Journal of Anatomy, 211, 567-76.

Novel cell culture device enabling three-dimensional cell growth and improved cell function.

Bokhari, M., Carnachan, R., Cameron, N.R., Przyborski, S.A. (2007).
Biochemical and Biophysical Research Communications, 354, 1095-1100.

Tailoring the morphology of emulsion-templated porous polymers.

Carnachan, R.J., Bokhari, M., Przyborski, S.A., Cameron, N.R. (2006).
Soft Matter, 2, 608-616.

Porous polymers by emulsion templating.

Barbetta, A., Carnachan, R.J., Smith, K.H., Zhao, C., Cameron, N.R., Kataky, R., Hayman, M., Przyborski, S.A., Swan, M. (2005).
Macromolecular Symposia, 226, 203-211.

Growth of human stem cell-derived neurons on solid three dimensional polymers.

Hayman, M.W., Smith, K.H., Cameron, N.R., Przyborski, S.A. (2005).
Journal of Biochemical and Biophysical Methods, 62, 231-240.

Enhanced neurite outgrowth by human neurons grown on solid three-dimensional scaffolds.

Hayman, M.W., Smith, K.H., Cameron, N.R., Przyborski, S.A. (2004).
Biochemical and Biophysical Research Communications, 314, 483-488.